U91
HUGO Gene Nomenclature Committee
HGNC Approved SymbolHGNC Approved Name
SCARNA17small Cajal body-specific RNA 17
snoid : SR0000257
Length : 83
Abstract : Homo sapiens U91 snoRNA. This RNA was cloned from HeLa cells by Darzacq et al. (2002) and is the orthologue of MBII-119 cloned by Hüttenhofer et al. (2001). It is predicted to guide the 2'O-ribose methylation of U4 snRNA C8. By FISH experiments, U91 was localized in Cajal bodies. It was later characterized as mgU4-8 by Tycowski et al. (2004), and localized in the nucleolar fraction. mgU4-8 is a part of a larger guide RNA, mgU12-22/U4-8 (see this entry), that resides in the nucleoplasmic fraction, in agreement with a localization in Cajal bodies. It is therefore possible that the localisation of U91 in Cajal bodies by FISH was attributable to the hybridisation of the probe with its precursor mgU12-22/U4-8. After the overexpression of U91 in HeLa cells, FISH experiments revealed a nucleolar and Cajal body localisation, suggesting that the mature U91 indeed resides in the nucleolus (P. Richard and T. Kiss, personal communication).
GenBank accession number : AY077741
Host gene : Independent transcriptional unit
Click here to see the position on the UCSC Genome Browser
Target RNA : U4 snRNA C8
sno/scaRNAs with same target U4 snRNA C8 : mgU12-22/U4-8   
References :
- Darzacq, X., Jady, B. E., Verheggen, C., Kiss, A. M., Bertrand, E., and Kiss, T. (2002). Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs. Embo J 21, 2746-2756.
- Huttenhofer, A., Kiefmann, M., Meier-Ewert, S., O'Brien, J., Lehrach, H., Bachellerie, J. P., and Brosius, J. (2001). RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse. Embo J 20, 2943-2953.
- Tycowski, K. T., Aab, A., and Steitz, J. A. (2004). Guide RNAs with 5' Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa. Curr Biol, 14, 1985-1995.
Sequence :
snoRNABase - http://www-snorna.biotoul.fr/