| mgU12-22/U4-8 | |||||||
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| snoid : SR0000256 | ||||||
| Length : 421 | ||||||
| Abstract : Homo sapiens mgU12-22/U4-8 scaRNA. This scaRNA was characterized by Tycowski et al. (2004). It is composed of two C/D box domains that are predicted to guide the 2'O-ribose methylation of U12 snRNA G22 and U4 snRNA C8. The 3' C/D box domain was previously cloned by Darzacq et al. (2002) and named U91 (see this entry). Northern blot analysis of RNAs from HeLa cells revealed the presence of the full length mgU12-22/U4-8, as well as U91 (Tycowski et al., 2004). Cell fractionation experiments localized mgU12-22/U4-8 in the nucleoplamic fraction (in agreement with a localization in Cajal bodies), but U91 in the nucleolar fraction. From its genomic localization, mgU12-22/U4-8 is transcribed from an independent unit. In agreement, a significant fraction bears a trimethylguanosine cap. | ||||||
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| GenBank accession number : BK005569 | ||||||
| Host gene : Independent transcriptional unit | ||||||
| Click here to see the position on the UCSC Genome Browser | ||||||
| Target RNA : U4 snRNA C8 and U12 snRNA G22 | ||||||
| sno/scaRNAs with same target U4 snRNA C8 : U91 | ||||||
| References : - Darzacq, X., Jady, B. E., Verheggen, C., Kiss, A. M., Bertrand, E., and Kiss, T. (2002). Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs. Embo J 21, 2746-2756. - Tycowski, K. T., Aab, A., and Steitz, J. A. (2004). Guide RNAs with 5' Caps and Novel Box C/D snoRNA-like Domains for Modification of snRNAs in Metazoa. Curr Biol, 14, 1985-1995. | ||||||
| Sequence : | ||||||
| snoRNABase - http://www-snorna.biotoul.fr/ | |||||||