Homo sapiens ACA31 snoRNA
This H/ACA box RNA was cloned by Kiss et al. (2004) from a HeLa cell extract immunoprecipitated with an anti-GAR1 antibody. It is predicted to guide the pseudouridylation of U3713 in 28S rRNA and U218 in 18S rRNA. The pseudouridylation of U3713 in 28S was previously reported by Ofengand and Bakin (1997). Maden and Wakeman (1988) could not determine whether U218, U219 or U220 of 18S rRNA was modified. The pseudouridylation of residue U218 was experimentally confirmed by primer extension mapping by Kiss et al. (2004).